Your e-stop will always be active (it cannot be turned off). However, if you are having problems resetting(the big red button on the main screen and other screens) go to the e-stop again in the configuration inputs and check or uncheck(one way or the other) the "active low" box, save, and try again. You should be able to activate Mach3 then....if this is what problem you are experiencing.
P.S. Let us know if this gets you further
I went back to the online video tutorials and found that the first part of the configuration is actually in the first, installation video. That certainly helped me understand that I could start with a really clean initial setup and not have to setup any limit switch items. That helped take some confusion out.
I checked the Estop "active low" box but still got nowhere. When I use the cursor keys and Pgup and Pgdown keys, the DRO's move just fine now but nothing from the motors. I also saw someone on the CNC Zone forum was at his wits end trying to do the same thing as me - misery loves company.
So, at this point, my old Mach 2 installation still works fine. My new Mach3 configuration is very clean - motor pin settings only + Estop set to "ActiveLow". No LED's lit in the wrong place on the diagnostics screen that I can see. Motors are still stone, cold, dead though.
Is it possible that my Mach2 installation is interfering with Mach3 ? Please say "no" because if I uninstall Mach2 I won't have a working mill unless I reinstall.
Don't give up on me.
G'night John Bloy