I am saying to remove the cable and test with the volt meter at the port. The port will still output just you wont have anything connected except the volt meter. Your motors etc wont run but you will see changing voltages on your meter, assuming the port is working.
As I said you will likely have to change the active state of limits and E-Stop for you to be able to take Mach out of Reset when you have the cable disconnected from the port, that will only take a few seconds to do and you can change back once everything gets hooked up again.
Regarding the Enables, they are not used to enable these drives, the 5v applied disables them, not a great way of doing things as normally you would take away the 5v to disable and apply to enable, it is done that way for safety.
Lights on Diagnostics page? I am not sure which ones you mean but can you also tell me, do you have a frequency and time in int on Diagnostics page and is it a 32bit operating system you are using?
Hood