It has been my understanding that Mach3 would run on any PC using an USB drive with a working copy of Mach3mill from the PC that had been running the CNC before on it.
If you don't run the Mach3 installer, the parallel port driver will not be installed. I beleive that this is why the motor tuning window does not display.
What you want to do is first install Mach3 using the installer, and then copy the entire Mach3 folder from your flash drive and overwrite the one you just installed.
This will give you a functioning duplicate of the install on the other machine.
As for the port address, you only need to enter C400.