More information on the issue:
I upgraded because I had problems with toolpath generation and M0 instructions, this is the recommendation in the thread about that issue in this forum. I decided to upgrade to the latest buid.
I made some more investigation on my machine and found the following:
1- If I activate the single block before start running a program it does not work
2- If I click single block in the middle of the execution of the program, the execution stops at the next instruction and single block works as expected.
Using Mach4 with stock router window and pokeys plugin.