Unfortunately, I think a re-install is the only way forward.
This is the stock answer:-
'If you click on Mach3 Menu -> Config -> Motor Tuning and it just won't open, but everything else works, you will need to reinstall Mach3 again.
Something in Mach3 or the registry in Windows for Mach3 got messed up. You should not need to do anything other than rerun the installer for Mach3 and then it should just work the next time you run Mach3. That being said, it would be a prudent idea to make a copy of your entire "C:\Mach3\" folder first, just in case something goes wrong'.
Tweakie.